This method employs diazirine-based compounds that, upon exposure to UV light (~365 nm), generate highly reactive carbene intermediates. These intermediates form stable covalent bonds with surrounding molecules in a non-selective manner, making the technology particularly suitable for molecular interaction studies and functional research.
Advantages
High reactivity
The generated carbene intermediate is highly reactive and can form covalent bonds with a variety of molecules.
Small molecule size
The diazirine molecule is small and has little effect on the structure and function of the target molecule.
Wide applicability
Applicable to a variety of biomacromolecules and materials.
