Nanocapture® Sensor Chips
Nanocapture® Sensor Chips are specialized sensors with application specific surface chemistries for immobilizing array content for analysis using the PlexArray® HT.
Nanocapture Sensor Chips consist of a gold-layered surface compatible with most commercially available protein microarray printers. Following printing of user-defined content, a specially designed Nanocapture® Sensor Chip Cover is attached to create a flow chamber through which sample analyte and processing reagents are passed. Binding events are then detected and monitored via surface plasmon resonance.
Advantages of Nanocapture® Sensor Chips:
- Flow cell sample volume of 30 uL
- Application flexibility with open application and specific surface application chemistries
- Low, non-specific binding providing high signal-to-noise ratios
- Flexible array density for low to high density array applications
- Selectable array spot size for application optimization
- Plug-and-Play functionality with the PlexArray® HT
Plexera's Nanocapture® Surface Chemistries
Nanocapture Gold
- Allows for easy immobilization of a multitude of proteins and antibodies
- Minimal Surface Preparation
- Low cost
Nanocapture SAM
- Two-dimensional surface chemistry that utilizes functional-group-terminated polymer chains
- Reduces background binding when compared to Nanocapture Gold
- Enables the use of customized linker groups, which increases the versatility of the surface
- Directionally immobilizes and orients ligands for increased accuracy in kinetic fitting
- Compatible with a wide range of ligands
Nanocapture SIP
- Utilizes three-dimensional polymer brush chains to achieve increased immobilization capacity
- Leads to increased signal/sensitivity
- Compatible with a wide range of ligands
Nanocapture DT
- Hydrogel surface density can be tailored to the application
- Assembled using controlled densities of dextran chains
- Three-dimensional surface provides increased immobilization capacity
- Retains ligand compatibility of Nanocapture SAM and Nanocapture SIP